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KMID : 0895820140240010031
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 1 p.31 ~ p.45
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Effects of Leejung-tang (理中湯) on MIA-Induced Osteoarthritis Rat
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Kook Gil-Ho
Oh Min-Seok
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Abstract
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Objectives : This study intends to clarify how Leejung-tang (here in after reffered to LJT) affect Wistar Rat whose osteoarthritis was induced by MIA.
Methods : Osteoarthritis was induced into rat by injecting MIA in its knee joint. Rats are divided into a total of 4 groups (n=6). Normal group are not treated at all without inducing osteoarthritis whereas control group were induced for osteoarthritis by MIA and oral medi-cated with 2 ml of physiological saline per day. Positive comparison group (Indomethacin) was injected with MIA and after 7 days, 2 mg/kg of Indomethacin was medicated. Experimental group (LJT) was injected with MIA and after 7 days that was medicated with 23 mg/kg of LJT. Indomethacin and LJT were oral medicated for each substance a total of 4 weeks with one time per day. After experiments (from 1 week after injection of MIA to 4 weeks elapsed), Hind paw weight bearing ability, Functions of liver and kidney, Serum prostaglandin E2, TNF-α, IL-1β, IL-6, Osteocalcin, TIMP-1, MMP-9, LTB4 and amount of cartilage were measured and histopathological variations for knee joint structures were observed.
Results: 1) Hind paw weight bearing ability of LJT administration group was increased but there was no statistical significance. 2) Functions of liver and kidney were not affected. 3) Serum prostaglandin E2, IL-1β, Osteocalcin, MMP-9 were significantly decreased and TNF-α, IL-6, TIMP-1, LTB4 were also decreased but there were no statistical significance. 4) In H&E staining and Safranin-O staining, there were small histopathological changes in LJT administration group than control group. 5) In micro CT (computed tomography)-ar-thrography, cartilage destruction was more suppressed in LJT administration group than control group.
Conclusions : Based on all results mentioned above, Leejung-tang (LJT) is believed to be meaningful for suppressing the progress of osteoarthritis and its treatments because of its anti-inflammatory effects and alleviation of pain with histopathological effective efficacy.
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KEYWORD
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Leejung-tang, LJT, Osteoarthritis, MIA-induced osteoarthritis, Cytokine, Indomethacin
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